Vector IG Sequence Link :
General : plasmid ds-DNA 2770 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)(E.coli JM109)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. Deposited by: J. Justin McCormick Cloning vector with the multiple cloning site expanded by inserting an oligonucleotide at the SmaI site of pUC19, thereby generating an amber mutation in the lacZ' coding sequence. [1] The plasmid contains the following restriction sites (bp from 0): DrdI--91, 992; KasI--233; PvuI--276, 2150; EcoRI--396; NotI--418; NsiI--493; HindIII--531; SapI--767; BsaI--1850; SspI--2585. (personal communication) The order of the major features in this plasmid is: KasI - 3' lacZ'amb1 - EcoRI/MCS/HindIII - 5' lacZ'amb1 - SapI - pMB1 ori - ampR. [1] Restriction digests of the clone give the following sizes (kb): PvuI--1.9, 0.9; EcoRI--2.8; HindIII--2.8; NotI--2.8; SspI--2.8. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pUC19)
Siblings : ()
Descendents : ()

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