pS5


Vector IG Sequence Link :
General : plasmid ds-DNA 2714 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)(E.coli JM109)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. Deposited by: J. Justin McCormick Cloning vector with a different multiple cloning site in lacZ', generating an amber mutation in the lacZ' coding sequence. [1] Derived from pS1 (ATCC 77381) by deletion of some restriction sites. [1] The plasmid contains the following restriction sites (bp from 0): DrdI--91, 932; KasI--233; BglI--245; PvuI--276, 2090; NruI--209; HindIII--471; SapI--707; EarI--708; BsaI--1790; SspI--2525. (personal communication) The order of the major features in this plasmid is: KasI - 3' lacZ'amb5 - NruI/MCS/HindIII - 5' lacZ'amb5 - SapI - pMB1 ori - ampR. [1] Restriction digests of the clone give the following sizes (kb): PvuI--1.8, 0.9; BglI--1.6, 1.1; HindIII--2.7; SspI--2.7; NruI--2.7. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pUC19)
Siblings : ()
Descendents : ()


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