Vector IG Sequence Link :
General : plasmid ds-DNA 9000 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : A derivative of pNKY51 with kanR from Tn903 inserted at the SmaI site of pNKY51. (personal communication) The order of the major features in this plasmid is: BglII (nt 1) - hisG - nt 1150 - URA3 - nt 2350 - kanR - nt 3850 - hisG - BamHI (nt 5000) - pMB1 ori - ampR. (personal communication) Restriction digests of the clone give the following sizes (kb): BglII--9.0; BamHI--9.0; BamHI/BglII--5.2, 3.8. (ATCC staff) A plasmid containing an excisable URA3 cartridge for gene disruption that permits repeated use of URA3 selection in constructing multiply disrupted yeast strains. Used by inserting the excised cassette into a cloned target gene, isolating a restriction fragment containing the cassette with flanking target gene sequences, transforming Ura3- strains, & selecting for stable integrants. Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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