Vector IG Sequence Link :
General : plasmid ds-DNA 9000 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : YC-type shuttle enhancer-cloning vector using beta-galactosidase activity as the reporter. (personal communication) The order of the major features in this plasmid is: MCS - CYC - lacZ - pMB1 ori - ampR - URA3 - ARS1 - SUP11 - CEN4. (personal communication) The UAS has been deleted from the CYC1 promoter, so expression of a CYC1-lacZ fusion protein depends on UAS elements cloned into polylinker region. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; XhoI--9.2; SalI--9.2. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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