pSHT


Vector IG Sequence Link :
General : phagemid ds-DNA 4000 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)(vertebrate cells)
Suppliers : (ATCC)
Misc.Comments : Deposited by: Phillip Bird Shuttle expression vector providing a start codon and signal sequence (from influenza virus hemagglutinin HA) necessary to express membrane and secretory proteins in mammalian cells. [1] Contains stop codons in all three frames after cloning sites. [1] The order of the major features in the phagemid is: pMB1 ori - ampR - M13 ori - ClaI - SV40 ori - SV40 early promoter - T7 promoter - HindIII - HA signal sequence - BamHI/SmaI/SpeI - termination signals - EcoRI - NheI- SV40 intron - BclI - SV40 polyadenylation. [1] Restriction digests of the clone give the following sizes (kb): SmaI--4.0; BamHI--4.0; HindIII--4.0. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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