pSI4


Vector IG Sequence Link :
General : plasmid ds-DNA 11900 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(Saccharomyces cerevisiae)
Suppliers : (ATCC)
Misc.Comments : Consists of the entire 2 micron circle genome cloned, at the EcoRI site of the small unique region, into the EcoRI site of pBR322. The leu2-d sequence (approximately 1.4 kb) is inserted into the PstI site of the 2 micron circle. [2] High-copy YE-type shuttle vector. [2] Restriction digests of the clone give the following sizes (kb): BamHI--12.0; EcoRI--4.4, 4.1, 2.6, 0.8; HindIII--4.6, 3.4, 2.7, 1.3; PstI--12.0. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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