Vector IG Sequence Link :
General : plasmid ds-DNA 4007 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli LK111)(E.coli)
Suppliers : (ATCC)
Misc.Comments : A high-copy-number cloning vector permitting visual detection of recombinants using insertional inactivation of the Rhodococcus gene for indigo production. Includes priming sites useful for sequencing. [1] The indigo pigment is intracellular and is produced on unsupplemented LB agar. Production is subject to catabolite repression by glucose, but is enhanced by tryptophan, indole, lactose or IPTG. [1] The M13 reverse sequencing primer and a 17mer primer [(5'-3') (CGAAGGCCGGTACCAGG)] complementary to the 5' end of the pigment gene (nt 849-832) can be used for sequencing cloned DNA inserts. [1] The portion of the indigo-producing gene retained in the construct corresponds to nt 791-2109 of the reported sequence. [1] The order of the major features in this plasmid is: lacOP - EcoRI/MCS/SphI - indigo - lacZ' remnant - ampR - pMB1 ori - lacI. [1] Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--2.7, 1.4; EcoRI--4.1; BamHI--4.1. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


Return to Vector Homepage