Vector IG Sequence Link :
General : plasmid ds-DNA 7503 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. Constructed by ligating pBR322 and pE194 after linearizing with PstI. The EcoRI site was destroyed and a HpaI/SstI fragment containing ErmC was removed. The ErmC promoter and ribosome binding sequence was inserted upstream of an EcoRI linker. The EcoRI cloning site is 4 bp downstream of the ribosome binding sequence. pSM6 can be maintained in both E.coli and B. subtilis but can be used as a cloning vector only in E.coli because the tetracycline resistance is poorly expressed in B. subtilis. Medium is 1273 LB plus tetracycline.
Parents : (pBR322)(pE194)
Siblings : ()
Descendents : ()

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