pSP2


Vector IG Sequence Link :
General : plasmid ds-DNA 5580 BP
Functions : (cloning)(sequencing)(shuttle)(RNA synthesis in vitro)
Selection : ()
Copy Number :
Hosts : (Schizosaccharomyces pombe)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Shuttle vector transforming S. pombe at high efficiency. Suitable for generating nested deletions, DNA sequencing, and producing riboprobes. Permits visual detection of recombinants by lacZalpha complementation. Constructed from pRS306 by inserting a 1.2 kb EcoRI fragment containing ars1 into the AatII site. The NdeI site in ars1 was removed. The order of the major features (with directions of transcription) is: > URA3 - f1 - < lacZ' - SacI/MCS/KpnI - pMB1 ori - < ampR - ars1. [1] Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: G.F.Cottarel
Parents : ()
Siblings : ()
Descendents : ()


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