Vector IG Sequence Link :
General : plasmid ds-DNA 5500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)
Suppliers : (ATCC)
Misc.Comments : Deposited by: T. Subramanian Shuttle expression vector permitting levels of protein production in human cells high enough for immunoprecipitation. [1] Contains two mammalian transcription units: the foreign DNA regulated by HIV-1 LTR and an SV40 polyadenylation sequence, and HIV-1 tat and neo regulated by an SV40 early promoter and polyadenylation sequence. [1] The AflII and SpeI cloning sites are recommended for the 5' end of foreign DNA to prevent potential translation effects of ATG sequences in the multiple cloning site. [1] The order of the major features in this plasmid is: AatII - HIV-1 LTR - MCS - SV40 polyadenylation - SV40 early promoter - tat - neoR - SV40 polyadenylation - pMB1 ori - ampR. [1] Restriction digests of the clone give the following sizes (kb): HindIII--5.5; NotI--5.5; KpnI--5.5. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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