Vector IG Sequence Link :
General : plasmid ds-DNA 5500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): HindIII/BglII--3.87, 1.34, 0.69; PvuII/PstI--3.66, 1.84; PvuII--uncut. (ATCC staff) Consists of the SV40 promoter (SV nt 270-5171), a HindIII linker, the hph gene (1347 bp), SV40 splice site (SV nt 4710-4100), SV40 polyadenylation site (SV nt 4362-2067), (personal communication from Paul Berg, Marianne Dieckmann) pML (inserted at the BamHI site with HindIII-EcoRI-amp upstream of the SV40 promoter). (personal communication from Paul Berg, Marianne Dieckmann) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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