Vector IG Sequence Link :
General : plasmid ds-DNA 6894 BP
Functions : (expression)(reporter)(promoter analysis)
Selection : ()
Copy Number :
Hosts : (E.coli DH5alpha)(E.coli)(monkey COS-1 or COS-7 cells)(vertebrate cells)
Suppliers : (CLONTECH)(ATCC)
Misc.Comments : Recommended ampicillin concentration is 200 ug/ml. (personal communication) The order of the major features in this plasmid is: pUC19 - EcoRI - SV40 enhancer - SV40 early promoter - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - HindIII - pUC19. [1] Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining or fluorometric assay. [1] Designed to express high levels of beta-galactosidase or an alternative gene product in COS cells. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors. [1] The lacZ gene may be excised by NotI and replaced with alternative sequences. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--7.0; HindIII--7.0; EcoRI/HindIII--4.3, 2.7; NotI--3.6 (doublet); BamHI--3.6, 3.2, 0.2. (ATCC staff) Medium is -1 TB plus ampicillin (200 ug/ml).
Parents : (pBR322)
Siblings : (pADbeta)(pNASSbeta)(pCMVbeta)(pTKbeta)
Descendents : ()

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