pT


Vector IG Sequence Link :
General : plasmid ds-DNA 5423 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)(E.coli JM105)(E.coli JM109)(vertebrate cells)
Suppliers : (ATCC)
Misc.Comments : Deposited by: J. Justin McCormick The first site was designed for insertion of a marker selectable in mammalian cells, and the second for the sequence to be expressed. The first site permits detection of inserts by alpha complementation. [1] Expression vector containing two multiple cloning sites: the first interrupting the lacZ' gene, and the second being flanked by a murine metallothionein I promoter and splicing and polyadenylation signals. [1] The plasmid contains the following restriction sites (bp from 0): NotI--0; EcoRI--9, 2894; SacI--1593; StyI--1627; ClaI--1814; XhoI--1836; BglII--1859; SphI--2730; NsiI--2732; PvuI--2774, 4564; HindIII--2945; DrdI--3406, 5277; BsaI--4264. (personal communication) The order of the major features in this plasmid is: NotI - 5' metallothionein promoter 3' - ClaI/MCSII'/BglII - poly(A) - SphI - 3'lacZ' - EcoRI/MCSI/HindIII - 5' lacZ' - pMB1 ori - ampR. [1] Restriction digests of the clone give the following sizes (kb): PvuI--3.6, 1.8; EcoRI--2.9, 2.5; HindIII--5.4; BglII--5.4; ClaI--5.4. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


Return to Vector Homepage