pT81luc


Vector IG Sequence Link :
General : plasmid ds-DNA 6100 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): BamHI--6.6; HindIII--6.6; SalI--6.6; matches depositor's DNA. (ATCC staff) Restriction digests of the clone give the following sizes (kb): BamHI--6.6; HindIII--6.6; SalI--6.6; matches depositor's DNA. (ATCC staff) This contains bp -81 to +52 of the herpes simplex tk promoter; distal GC + CCAAT have been deleted. [1] This is a derivative of plasmids patented by the University of California and is not to be used for commercial purposes without prior arrangement with the University of California. (personal communication) There is cryptic promoter activity which may be more readily observed with weak promoters. Be careful to do all appropriate controls and map the transcripts. (personal communication) One of a series of luciferase vectors (ATCC 37574-37584) for assaying promoter activity in mammalian cells. [1] Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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