pTacNeo2.2


Vector IG Sequence Link :
General : plasmid ds-DNA 5440 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli DG101)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Translation of LacZ is in frame with APH(3')-I. (personal communication) Sequences from the TAA stop codon of APH(3')-I to the immediate downstream PvuII site encode a portion of Tn601. Sequences from the PvuII site to the PstI site are derived from Tn5. (personal communication) The start codon of the modified APH(3')-I is followed by the eleventh codon (serine) of APH(3')-I. Codons 2-10 have been deleted. (personal communication) The vector contains pBR322 (EcoRI-SphI), a TAC promoter, the first 8 amino-terminal codons of beta-galactosidase, & a gene encoding a modified aminoglycoside phosphotransferase (3')-I (APH(3')-I). (personal communication) This is an expression vector capable of producing a beta-galactosidase: insert: APH(3')-I fusion protein. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI/SphI--3.9, 1.3, 0.3; PvuII--3.9, 1.6; PstI--3.1, 2.4. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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