pTAG-1


Vector IG Sequence Link :
General : phagemid ds-DNA 6000 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)(vertebrate cells)
Suppliers : (ATCC)
Misc.Comments : Deposited by: Judith L. Fridovich-Keil The order of the major features in this plasmid is: SalI - SV40 polyadenylation trimer - XhoI/MCS/HindIII - beta-globin - PstI - SphI - ApaI - ampR/pGEM-7Zf(+). [1] Contains a trimer cassette of the SV40 major late polyadenylation signal to block background readthrough expression of the reporter gene. [1] A promoter/enhancer-cloning shuttle vector using human beta-globin as the reporter gene. Primarily useful for analysis of gene expression at the RNA level. [1] The ApaI site can be used for cloning enhancer elements. [1] Restriction digests of the clone give the following sizes (kb): PstI--3.8, 2.2; SphI--3.8, 2.2; HindIII--6.0; XhoI--6.0; BamHI--5.5, 0.55. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pbetaGlo4pA from pbetaGlo, from pbetaGloXH from pGEM7, & pJFCAT1)(pBLCAT3)
Siblings : ()
Descendents : ()


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