pTB


Vector IG Sequence Link :
General : plasmid ds-DNA 5846 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)(E.coli JM105)(E.coli JM109)(vertebrate cells)
Suppliers : (ATCC)
Misc.Comments : Deposited by: J. Justin McCormick The first site was designed for insertion of a marker selectable in mammalian cells, and the second for the sequence to be expressed. [1] Both sites permit detection of inserts by alpha complementation, with discrimination between the two provided by the amber mutation in the second lacZ. [1] Expression vector containing two lacZalpha-based multiple cloning sites, with the second containing an amber mutation and being flanked by a mouse metallothionein I promoter at one end and splicing and polyadenylation signals at the other. [1] The plasmid contains the following restriction sites (bp from 0): NotI--0; EcoRI--9, 3272; SacI--1593; PvuI--1842, 3197,4987; ClaI--1981; XhoI--2005; BglII--2028; SphI--3153, 3356; NsiI--3155; HindIII--2040,3368; DrdI--3829, 5700; BsaI--4687. (personal communication) The order of the major features in this plasmid is: NotI - 5' metallothionein 3' - 3' lacZ'amb5 - NruI/MCSII''/HindIII - 5' lacZ'amb5 - splicing - polyadenylation - NsiI - 3' lacZ' - EcoRI/MCSI/HindIII - 5' lacZ' - pMB ori - ampR. [1] Restriction digests of the clone give the following sizes (kb): PvuI--2.6, 1.8, 1.4; EcoRI--3.3, 2.5; HindIII--4.5, 1.3; BglII--5.8; ClaI--5.8. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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