Vector IG Sequence Link :
General : plasmid ds-DNA 5600 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Trypanosoma cruzi)(Leishmania)(E.coli)(E.coli SURE)(E.coli XL-1)(Leishmania mexicana)(Leishmania donovani)
Suppliers : (ATCC)
Misc.Comments : Shuttle expression vector for Trypanosoma cruzi and Leishmania. The neoR gene can be used as a selectable marker in T. cruzi and Leishmania. Increasing the concentration of G418 in the media increases copy number and expression. Transfected DNA is maintained in transformed parasites extrachromosomally as circular head-to-tail tandem repeats. The order of the major features in this plasmid is: SacI - SacII - 5' flanking region of gGAPDH I - SpeI/MCS/XhoI - PstI - gGAPDH I/gGAPDH II intergenic region - neoR - gGAPDH II 3' flanking region - KpnI - ampR - pBluescript KS. DNA can be rescued from transformed parasites and propagated in E.coli after linearizing and religation to produce single circular copies. Restriction digests of the clone give the following sizes (kb): PstI--4.8, 0.65, 0.2; BamHI--5.6; EcoRI--5.6; KpnI--5.6. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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