pTF1


Vector IG Sequence Link :
General : phagemid ds-DNA 3043 BP
Functions : (cloning)(sequencing)(mutagenesis)(transcription)
Selection : (color blue/white)
Copy Number :
Hosts : (E.coli JM103)(E.coli NM522)(E.coli DH5alpha)(vertebrate cells)(HeLa T4+ cells)
Suppliers : ()
Misc.Comments : pTF1 requires a reverse primer. This entry [NCBI gibbsq 122487] was created by the journal scanning component of NCBI/GenBank at the National Library of Medicine. This sequence comes from Fig. 1C. Deposited by: Allen Portner High-copy-number plasmid (in bacteria) with increased efficiency of liposome-mediated introduction into cultured cells. [1] Can express foreign sequences in cells producing T7 RNA polymerase, such as that encoded by recombinant vaccinia virus vTF7-3 ATCC VR-2153. [1] Constructed by inserting the 152 bp BamHI-BglII fragment from pAR2529 into the BamHI site of pTZ18R. Polylinker sites were removed and were replaced with the 48 bp EcoRI-KpnI fragment of pBluescript KS+. [1] The order of the major features of the plasmid is: universal primer sequence - XbaI - T7 phi 10 terminator < - XbaI - BamHI/MCS/EcoRI - < T7 phi 10 promoter - reverse primer sequence - pMB1 ori - < ampR - f1 ori.[1]CC A correction to the published sequence has been submitted. Sequence 2173-2252 should read: GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG. (personal communication) Restriction digests of the clone give the following sizes (kb): BamHI--3.0; EcoRI--3.0; HindIII--3.0. (ATCC staff) Medium is 1227 LB plus ampicillin. Sequence comes from Fig. 1C. [1] NCBI gi: 263065
Parents : (pTZ18R)(pAR2529)(pBluescript II KS+)
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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