pTG12


Vector IG Sequence Link :
General : plasmid ds-DNA 37400 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli RR1)(Streptomyces)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Size may be 43400. This was constructed from pTG6 (ATCC 39079) by deleting a 6 kb fragment which includes the SphI site at 4.75 kb, the PstI site at 6.38 kb, and the BamHI site at 7.27 kb. The BamHI cloning site is at 9.39 kb. Maintained as plasmid in E.coli. When undigested plasmid DNA from E. coli is used to transfect protoplasts of Streptomyces cattleya, phage are generated. Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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