Vector IG Sequence Link :
General : plasmid ds-DNA 7481 BP
Functions : (expression)(reporter)(promoter analysis)
Selection : ()
Copy Number :
Hosts : (E.coli DH5alpha)(E.coli)(monkey COS cells)(vertebrate cells)
Suppliers : (CLONTECH)(ATCC)
Misc.Comments : ATCC size is 7600 bp. The order of the major features in this plasmid is: pUC19 - EcoRI - HSV thymidine kinase promoter - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19. [1] The lacZ gene may be excised by NotI and replaced with alternative sequences. [1] Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining. [1] Designed to express low levels of beta-galactosidase or an alternative gene product. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors. [1] Restriction digest of the clone give the following sizes (kb): EcoRI--7.2, 0.9; HindIII--7.6; EcoRI/HindIII--4.5,2.7,0.9; NotI--4.4, 3.2; BamHI--3.8 (doublet). (ATCC staff) Medium is -1 TB plus ampicillin (200 ug/ml).
Parents : (pBR322)
Siblings : (pADbeta)(pNASSbeta)(pCMVbeta)(pSVbeta)
Descendents : ()

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