pTN1060


Vector IG Sequence Link :
General : plasmid ds-DNA 10200 BP
Functions : (cloning)(protein Tra+)
Selection : ()
Copy Number :
Hosts : (Streptococcus lactis)
Suppliers : (ATCC)
Misc.Comments : Verified to be gram positive, nisR and to grow in chains. (ATCC staff) High frequency conjugal transfer plasmid, confering phage restriction and modification activity and lactose fermentation to group N Streptococci. pTN1060 confers the following phenotype: Tra+, Lac+, nisR, R/M, and Clu-. nisR indicates resistance to the antimicrobial protein nisin and Clu- indicates the absence of the clumping phenotype. Cell aggregation is not imposed on transconjugants. The plasmid contains the following restriction sites (approximate kb from the SmaI site unique in pTR1040):BamHI--51, 93; BglI--23, 71; KpnI--25, 36, 54, 57; PstI--15, 35, 59, 86, 101; PvuI--31, 61, 75; SalI--18, 100; SmaI--0, 83, 102. [1] Note: This material is cited in a U.S and/or other Patent and may not be used to infringe the patent claims.
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