pUC830


Vector IG Sequence Link :
General : phagemid ds-DNA 2710 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(E.coli)
Suppliers : (ATCC)
Misc.Comments : These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Expression vector useful for construction of cDNA libraries and containing priming sites useful for sequencing. Permits visual detection of recombinants by beta-galactosidase alpha peptide complementation. [1] pUC830 (ATCC 77218) and pUC931 (ATCC 77219) differ in the orientation of the polylinker. [1] E.coli transformed with this plasmid produce larger colonies when an insert is present in the polylinker than when there is no insert. (personal communication) Restriction digests of the clone give the following sizes (kb): PstI--2.7; HindIII--2.7; EcoRI--2.7; NheI--2.7. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pUC8)(M13tg130)(bGal130)
Siblings : (pUC931)
Descendents : (pOM2)(pOM4)(pOM8)

NCBI ENTREZ Link :


Return to Vector Homepage