pUFR027


Vector IG Sequence Link :
General : plasmid ds-DNA 9300 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli DH5alpha)(broad host range)(E.coli)(Xanthomonas)
Suppliers : (ATCC)
Misc.Comments : The order of the major features in this plasmid is: lacZalpha with MCS (pUC18) - oriW (pSa747, IncW) - parA+ (pUCD550) - aphA (pUC4Kan) - mob(P) (pLAFR3, Mob+). Shuttle vector permitting visual detection of recombinants. (ATCC staff) Uses interruption of lacZalpha. (ATCC staff) The partition locus from Agrobacterium plasmid pTAR stabilizes the plasmid in X. citri and X. campestris pvs. campestris, vesicatoria, malvacearum, and translucens grown in broth culture. Restriction digests of the clone give the following sizes (kb): EcoRI--9.4; PstI--6.8, 1.4, 1.25; SmaI--7.7, 1.9. (ATCC staff) Medium is 1799 LB plus neomycin (10 ug/ml).
Parents : ()
Siblings : ()
Descendents : ()


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