Vector IG Sequence Link :
General : plasmid ds-DNA 6700 BP
Functions : (cloning)(shuttle)(integrating)(rpsL ribosomal protein S12)
Selection : ()
Copy Number :
Hosts : (broad host range)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Suicide, gene replacement vector for Yersiniae and other gram-negative hosts that permits positive selection for loss of the plasmid after allelic exchange. The vector also contains cos sites and oriT, and can be used for cosmid cloning or conjugative transfer. RP6 is a suicide replicon in hosts not producing the pir protein. RpsL (streptomycin sensitive) is dominant to streptomycin resistance, so loss of the plasmid can identified by streptomycin resistance and ampicillin sensitivity. The order of the major features in the plasmid is: EcoRV - R6K ori - oriT - SalI - cos - rpsL - ampR. [2] Deposited by: S.C.Straley
Parents : ()
Siblings : ()
Descendents : ()

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