pUMSV0CAT


Vector IG Sequence Link :
General : plasmid ds-DNA 5500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)(E.coli TB1)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 2.0; SmaI--5.7. (ATCC staff) A 1023 bp SacI/XbaI fragment containing the poly(A) signal of mouse c-mos (UMS) was cloned into the NdeI site of pSV0cat. The NdeI site is 56 bp upstream of the SmaI cloning site. Constructed by J-P. Salier and K. Kurachi. The UMS sequences reduce background CAT transcription from the vector to levels approaching mock-transfected cells. [1] A shuttle vector for assessing weak transcriptional activity of a cloned promoter using a CAT reporter gene. Medium is 1227 LB plus ampicillin.
Parents : (pSV0-cat)(UMS)
Siblings : ()
Descendents : ()


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