pUN25


Vector IG Sequence Link :
General : plasmid ds-DNA 5900 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : YC-type shuttle vector useful for the sectoring-shuffle mutagenesis assay. Contains promoters for in vitro RNA synthesis and permits visual detection of recombinants by lacZalpha complementation. The order of the major features in this plasmid is: ampR - pMB1 ori - lacZ'/MCS - CEN4 - SUP11 - ARS1 - TRP1. Restriction digests of the clone give the following sizes (kb): SacI--6.2; EcoRI--6.2; BamHI--6.2; PvuII--5.8, 0.4. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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