pUN30


Vector IG Sequence Link :
General : phagemid ds-DNA 5300 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : YC-type shuttle vector permitting visual detection of recombinants by lacZ alpha complementation and production of ssDNA (in the same sense as lacZ mRNA). The order of the major features in this plasmid is: ampR - pMB1 ori - lacZ'/MCS - CEN4 - M13+ - ARS1 - TRP1. Restriction digests of the clone give the following sizes (kb): EcoRI--5.4; KpnI--5.4; HindIII--3.7, 1.7. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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