pUR18


Vector IG Sequence Link :
General : plasmid ds-DNA 5500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli DH5alpha)(Schizosaccharomyces pombe)(E.coli)
Suppliers : (ATCC)
Misc.Comments : The order of the major features in this plasmid is:ars1 - ura4 - HindIII/MCS/EcoRI/lacZ' - pMB1 ori - ampR. Constructed by inserting a 1.7 kb ClaI fragment containing ura4 and a 1.2 kb ClaI fragment containing ars1 into the NdeI site modified with a ClaI linker. Shuttle vector permitting visual detection of recombinants by beta-galactosidase alpha complementation. Restriction digests of the clone give the following sizes (kb): EcoRI--5.5; BamHI--5.5; PstI--5.5; HindIII--5.5. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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