Vector IG Sequence Link :
General : plasmid ds-DNA 5500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli DH5alpha)(Schizosaccharomyces pombe)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Shuttle vector permitting visual detection of recombinants by beta-galactosidase alpha complementation. The addition of NotI and SfiI sites to the MCS enables unidirectional exonuclease III deletions & facilitates the rescue in E. coli of plasmids where dimerization has occurred. The order of the major features in this plasmid is: ura4 - ars1 - SfiI/MCS/HindIII/lacZ' - pMB1 ori - ampR. Restriction digests of the clone give the following sizes (kb): EcoRI--5.5; BamHI--5.5; HindIII--5.5; PstI--5.5. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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