pUXV3


Vector IG Sequence Link :
General : plasmid ds-DNA 6000 BP
Functions : (cloning)(shuttle)(expression)
Selection : ()
Copy Number :
Hosts : (Ustilago maydis)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Shuttle expression vector using the Ustilago maydis gap (glyceraldehyde phosphate dehydrogenase) promoter to drive expression of the cloned insert. One of a series of vectors (ATCC 77463-77465 and 77468-77471) differing only in the selectable marker and the orientation of the gap promoter. The order of the major features in the plasmid is: Ustilago maydis ARS - ampR - pMB1 ori - 5' lacZ fragment - EcoRI - SacI - gap promoter -> BamHI <- cbxR - 3' lacZ fragment. [2] The sequence conferring resistance to carboxin (cbxR) is a mutation of an iron-sulfur protein subunit of the Ustilago maydis succinate dehydrogenase gene. [3] Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: J.A.Bruenn
Parents : ()
Siblings : ()
Descendents : ()


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