pVT65


Vector IG Sequence Link :
General : plasmid ds-DNA 6500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli JM109)(Haemophilus influenzae)(E.coli)(Haemophilus influenzae BC200)
Suppliers : (ATCC)
Misc.Comments : Precautions have not been taken to maintain the F' in the JM109 host. The F' is not required to use this plasmid. (personal communication) Cloning into the HincII, PstI, PvuI, ScaI, or SspI sites inactivates ampR. Cloning into the BalI or NcoI sites inactivates cmlR. The order of the major features in this plasmid is: pMB1 ori - ampR - pRSF0885 rep - cmlR. Shuttle vector. Restriction digests of the clone give the following sizes (kb): PstI--6.6; PvuI--6.6; SspI--3.7, 2.9; PvuII--6.2, 0.46; BamHI--4.0, 2.4. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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