Vector IG Sequence Link :
General : plasmid ds-DNA 7000 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli JM109)(Haemophilus influenzae)(E.coli)(Haemophilus influenzae BC200)
Suppliers : (ATCC)
Misc.Comments : Precautions have not been taken to maintain the F' in the JM109 host. The F' is not required to use this plasmid. (personal communication) Cloning into the HincII, PstI, PvuI, or ScaI sites inactivates ampR. Cloning into the SmaI site inactivates cmlR. The order of the major features in this plasmid is: pMB1 ori - ampR - pRSF0885 rep - cmlR. Shuttle vector. Restriction digests of the clone give the following sizes (kb): PstI--7.0; PvuI--7.0; SmaI--7.0; BamHI--4.7, 2.4. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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