pWH1266


Vector IG Sequence Link :
General : plasmid ds-DNA 8900 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Acinetobacter calcoaceticus)(E.coli)(Acinetobacter calcoaceticus BD4)
Suppliers : (ATCC)
Misc.Comments : Contains the following restriction sites (approximate kb from the EcoRI site): BamHI--0.38; PvuII--2.1, 6.6; AccI--0.65, 4.5, 5.7, 6.7; HincII--0.65, 3.7, 8.4. The 2.2 AccI/HincII fragment is essential for replication in A. calcoaceticus. The cryptic plasmid pWH1277 isolated from A. calcoaceticus lwoffi was linearized with PvuII and cloned into the PvuII site of pBR322. Ampicillin and tetracycline resistance are both expressed in A. calcoaceticus. Restriction digests of the clone give the following sizes (kb): BamHI--8.8; PvuII--4.4 (doublet); AccI--3.8, 2.9, 1.2, 0.9; HincII--4.6, 3.1, 1.1; EcoRI--8.8. (ATCC staff) Shuttle vector. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 208940
Parents : ()
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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