pWL102


Vector IG Sequence Link :
General : plasmid ds-DNA 10500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli DH5alpha F')(halobacteria)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Shuttle vector with demonstrated ability to transform Haloferax volcanii, Haloferax mediterranei (ATCC 33500), Halobacterium halobium, Haloarcula hispanica (ATCC 33960), and Haloarcula vallismortis (ATCC 29715). [1] The order of the major features in this plasmid is: NcoI - BamHI - HindIII - XbaI - KpnI - mevR - sphI - pMB1 ori - ampR - EcoRI - ClaI - HindIII - pHV2 fragment. [3] The 3.5 kb SphI/KpnI fragment conferring mevinolin resistance has been sequenced. The mutation in the hmgA gene is at position -29 relative to the transcription start site, and results in an increased level of transcription. [2] Restriction digests of the clone give the following sizes (kb): EcoRI--11.0; HindIII--6.6, 4.1; KpnI/SphI--7.2, 3.6; PstI--7.6, 3.0; XbaI--11.0. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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