pXC47


Vector IG Sequence Link :
General : plasmid ds-DNA 5780 BP
Functions : (cloning)(expression)
Selection : (14-3-3)
Copy Number :
Hosts : (E.coli)(E.coli TAP308)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; HindIII--5.8. (ATCC staff) One of 12 expression vectors (ATCC 86990-87001) designed to maximize expression from the lambda PL promoter and support cloning of PCR products. The vectors differ in cloning sites and in translational enhancer and initiation sequences. The vector contains a 0.7 kb bovine cDNA that can be excised using the 5' and 3' cloning sites and be replaced by a gene of interest. The bovine insert may be used as a positive control for expression. The insert is expressed as a Met-His fusion protein. The lambda R1 terminator is partially deleted. The order of the major features of the plasmid is: pMB1 ori - ampR - EcoRI - lambda PL promoter - translational enhancer - synthetic SD sequence - 5' cloning site(s) - cDNA - 3' cloning sites. [1] Growth: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: Patterson T.A.
Parents : ()
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Descendents : ()


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