Vector IG Sequence Link :
General : phagemid ds-DNA 3753 BP
Functions : (cloning)(expression)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)
Suppliers : (USB)(ATCC)
Misc.Comments : + makes non-coding strand of gene. - makes coding strand of gene. pcDpolyB+ and pcDpolyB- differ in their orientations of the intergenic region from bacteriophage f1. Libraries constructed with each vector for a different cell type can be screened against each other for cell-specific sequences. A vector which maintains the cDNA cloning features of the Okayama-Berg vector while increasing the efficiency of making transformation-competent, cDNA-containing plasmids and allows enrichment by hybridization/selection. Allows expression of cloned cDNAs in eukaryotic cells from the SV40 early region promoter. Contains the intergenic region from bacteriophage f1 permitting synthesis of single-strand copies of the insert. Restriction digests of the clone give the following sizes (kb): KpnI--3.7; KpnI/HindIII--3.2, 0.5; BamHI--3.7; PvuII--uncut; PstI--3.7. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pBR322)(f1)
Siblings : (pXPRS-)
Descendents : ()

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