Vector IG Sequence Link :
General : yeast artificial chromosome ds-DNA 11454 BP
Functions : (cloning 50000-100000 bp)
Selection : (color red/white)
Copy Number :
Hosts : (E.coli)(Saccharomyces cerevisiae)(E.coli RR1)(Saccharomyces cerevisiae AB1380)
Suppliers : (Sigma)(ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. The HIS3 sequence is removed when producing the telomers by BamHI digestion. pYAC55 was constructed from pYAC2 by replacing the SmaI site in the SUP4 intron with the NotI site, & eliminating the SfiI and NotI sites flanking the SUP4 gene. The NotI site is located in the SUP4 intron and inserts inactivate expression. Medium is 1227 LB plus ampicillin.
Parents : (YIp5)(YCp19)(yeast sup4-o gene)(lambda)(yeast telomere)
Siblings : (pYAC4)
Descendents : ()


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