pYAC-RC


Vector IG Sequence Link :
General : yeast artificial chromosome ds-DNA 11487 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli MC1061)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. Due to ligation compatibility with sites in the polylinker, DNA fragments from BssHII, EagI, NaeI, NarI, NruI, SmaI and XhoI digests may also be cloned in this vector. Constructed by removing the ClaI and SalI sites from pYAC3 and inserting a polylinker in the SnaBI site within SUP4. SUP4 still functions. A yeast artificial chromosome vector for genomic library construction using DNA digested with infrequent restriction enzymes. Restriction digests of the clone give the following sizes (kb): HindIII--3.7, 3.0, 1.9, 1.47; SalI--12.0. (ATCC staff) Digestion with HindIII gives the following sizes (kb): 3.5, 3.0, 1.9, 1.4(doublet), small fragment. If any telomere DNA has been deleted, the doublet will be resolved and a new band will appear. (personal communication) An occasional isolate will spontaneously delete small amounts of telomere sequence. Check by digesting the plasmid with HindIII. (personal communication) Medium is 1227 LB plus ampicillin.
Parents : (pYAC3)
Siblings : ()
Descendents : ()


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