pYe CEN3 30

Vector IG Sequence Link :
General : plasmid ds-DNA 6300 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli JA228)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Contains unique BamHI, BglII, XbaI, NruI, and SalI sites, with XbaI and BglII inactivating TRP1. (personal communication) Constructed by inserting a 627 bp BamHI/Sau3AI fragment containing CEN3 into the BamHI site of YRp7'. [2] Demonstrates mitotic stability increased over YRp7'; 60-90% of transformed S. cerevisiae J17 maintain the Trp+ phenotype after 20 generations. [2] Restriction digests of the clone give the following sizes (kb): BamHI--6.3; BglII--6.3; SalI--6.3; EcoRI--4.6, 1.7. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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