R67'


Vector IG Sequence Link :
General : plasmid ds-DNA 353 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)
Suppliers : ()
Misc.Comments : The sequence reported here is a modified version of the type II methotrexate-resistant dihydrofolate reductase (DHFR) gene, R67 (see seperate entry). The 3' end of the original sequence was modified to determine if a conserved region of the gene encodes the active site of the protein. After modification, trimethoprim resistance in E. coli was observed at a comparable level to the one obtained with the unmodified gene, thus supporting the idea of a conserved region. NCBI gi: 208217
Parents : (R67)
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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