YCp8phocat


Vector IG Sequence Link :
General : plasmid ds-DNA 9260 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli RR1)(Saccharomyces cerevisiae)(E.coli)(Saccharomyces cerevisiae SHY1)
Suppliers : (ATCC)
Misc.Comments : One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast. A 7285 bp BamHI/HindIII fragment of YCp8 containing URA3 and CEN6 was ligated to a 1975 bp BamHI/HindIII fragment of YRp7-32phocat (ATCC 37625). The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al. Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--7.2, 2.1; BglII--6.0, 2.6; EcoRI--7.2,1.5,0.6. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


Return to Vector Homepage