YEp13


Vector IG Sequence Link :
General : plasmid ds-DNA 10639 BP
Functions : (cloning)
Selection : ()
Copy Number : pMB1 25-80 copies/cell
Hosts : (E.coli C600)(E.coli K802)(E.coli)(S.cerevisiae)(Saccharomyces)
Suppliers : (ATCC)
Misc.Comments : YEp13 is mitotically stable in cir+ strains. [2] A YE type S. cerevisiae/Escherichia coli shuttle vector for cloning specific genes by transformation of yeast. Transforms leu2- yeast to Leu+ at 10E3 to 10E4 colonies per microgram of DNA. An EcoRI fragment containing the 2 micron origin was cloned into the EcoRI site of pBR322. The resulting plasmid was partially digested with PstI, and a PstI fragment containing yeast LEU2 cloned into the 2 micron PstI site. Restriction digests analyzed on agarose gels give the following sizes (kb): PstI--5.5, 4.0, 0.9; HindIII--10.7. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pBR322)(2micron plasmid B form)
Siblings : (YEp351)(YEp352)(YEp356)(YEp366)(YEp24)
Descendents : ()

NCBI Entrez Link:


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