Vector IG Sequence Link :
General : plasmid ds-DNA 5644 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli RR1)(S.cerevisiae)(E.coli)(Saccharomyces)
Suppliers : (ATCC)
Misc.Comments : pUC18 was digested with NdeI and blunt-ended by filling in. A 2.95 BamHI/XbaI fragment of pC4B was blunt-ended (filled in) and ligated to the pUC18. The 2.95 kb fragment of pC4B includes the LEU2 gene (nt 286-2256 in the final construct, where the A of the ATG of lacZ' is number 1) and the 2 micron replication system (nt 2257-3241). Restriction digests of the clone give the following sizes (kb): BamHI--6.0; XbaI--6.0; PstI--6.0; HindIII--6.0. (ATCC staff) This is a E.coli/yeast episomal shuttle vector with multiple restriction sites for cloning. (personal communication) Medium is 1227 LB plus ampicillin. NCBI gi: 295270
Parents : (pBR322)(2micron)
Siblings : ()
Descendents : ()


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