Vector IG Sequence Link :
General : plasmid ds-DNA 5181 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli RR1)(S.cerevisiae)(E.coli)(Saccharomyces)
Suppliers : (ATCC)
Misc.Comments : Constructed by filling in and blunt-end ligating pUC18 linearized at NdeI, a 1.4 kb RsaI fragment of the 2 micron circle, and a 1.1 kb HindIII/SmaI fragment containing URA3. A XbaI site in 2u and a PstI site in URA3 were removed. [1] The yeast genomic sequence is in nt 286-1383 (where the A of the ATG of lacZ' is number 1), the 2 micron sequence goes from nt 1384-2768, & pUC18 includes nt 2769-285. [2] Restriction digests of the clone give the following sizes (kb): BamHI--5.3; XbaI--5.3; PstI--5.3; HindIII--5.3. (ATCC staff) This is a E.coli/yeast episomal shuttle vector with multiple restriction sites for cloning. (personal communication) Medium is 1227 LB plus ampicillin. NCBI gi: 295271
Parents : (pBR322)(2micron)
Siblings : ()
Descendents : ()


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