Vector IG Sequence Link :
General : plasmid ds-DNA 7944 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(E.coli MC1061)
Suppliers : (ATCC)
Misc.Comments : NCBI gi: 416342 The sequence and reading frame of the multiple cloning sequence is: 5' GA ATT CCC GGG GAT CCG TCG ACC TGC AGC CAA GCT TGC GAT CCC 3'. The sequence begins with the first nucleotide of the MCS & ends with the CCC for amino acid 8 of beta-galactosidase. One of 3 promoter-cloning YE type shuttle vectors (ATCC 37725 - 37727) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-2; SmaI-3; BamHI-3; SalI-3; PstI-1; HindIII-2. Restriction digests of the clone give the following sizes (kb): PstI--8.0; EcoRI--8.0; BamHI--8.0; HindIII--8.0. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pBR322)(2micron)
Siblings : ()
Descendents : ()


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