Vector IG Sequence Link :
General : plasmid ds-DNA 7963 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(E.coli MC1061)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. Restriction digests of the clone give the following sizes (kb): EcoRI--8.0; BamHI--8.0; SalI--8.0; PstI--8.0. (ATCC staff) The sequence and reading frame of the multiple cloning sequence is: 5' GAA TTC GAG CTC GGT ACC CGG GGA TCC TCT AGA GTC GAC CTG CAG GCA TGC AAG CTT GCT CCC 3', from nucleotide 1 of MCS through CCC for amino acid 8 of beta-galactosidase. One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37731 to ATCC 37733) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-1; SacI-2; KpnI-2; SmaI-1; BamHI-1; XbaI-1; SalI-1; PstI-2; SphI-2; HindIII-1. The SacI site is not unique. Medium is 1227 LB plus ampicillin.
Parents : (YEp354)(pUC18)
Siblings : ()
Descendents : ()

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