Vector IG Sequence Link :
General : plasmid ds-DNA 8400 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(E.coli MC1061)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): HindIII--8.4; BamHI--8.4; PstI--8.4. (ATCC staff) The sequence and reading frame of the multiple cloning sequence is: 5' AA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CCC GGG TAC CGA GCT CGA ATT CCC AGC TTC GAT CCC3', from nucleotide 1 of MCS through CCC for amino acid 8 of beta-galactosidase. One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37740 to ATCC 37742) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-2; SphI-3; PstI-3; SalI-2; XbaI-2; BamHI-2; SmaI-3; KpnI-3; SacI-3; EcoRI-2. The EcoRI, KpnI, and SacI sites are not unique. Cloning into the HindIII, SphI, PstI, SalI, or XbaI sites leads to a TAG stop codon within the downstream XbaI site of the multiple cloning region. Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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