YEp436


Vector IG Sequence Link :
General : plasmid ds-DNA 13300 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : YE-type shuttle vector. (ATCC staff) The order of the major features in this plasmid is: LEU2-d - ori(2 micron) - BamHI site - SalI site - URA3 - ampR. High copy number (100-200 copies) can result from selection for Leu+. (personal communication) Restriction digests of the clone give the following sizes (kb): BamHI/XbaI--6.0, 4.5, 2.8; SalI--13.5; SmaI--13.5. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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