Vector IG Sequence Link :
General : plasmid ds-DNA 7800 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli MM294)(E.coli C600)(S.cerevisiae SHY series)
Suppliers : (ATCC)
Misc.Comments : A 1.1 kb HindIII fragment containing URA3 and a 2.2 kb SalI/XhoI fragment containing LEU2 were cloned into the HindIII and SalI sites, respectively, of pBR322. Unique restriction sites are in the 3' untranslated regions of URA3(SmaI) and LEU2(SalI). [2] Orientations are such that transcription from both URA3 and LEU2 is clockwise. [2] A general purpose integrating Saccharomyces cerevisiae/E.coli shuttle vector. [1] Restriction digests analyzed on agarose gels give the following sizes (kb): EcoRI--4.7, 3.2; BamHI--7.8. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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